负压二级生物安全实验室设计关键控制点分析
Critical control point design of negative pressure biosafety level-2 laboratories
摘要：
以两个负压二级生物安全实验室为例，分析了实验室的功能分区与平面布局、通风空调方式、气流组织、送排风系统等关键控制点的设计。建议核心工作间（动物室）划分相对清洁侧和相对污染侧；设置缓冲间、更衣室和准备间；通风空调采用全空气直流系统；整体气流由清洁区流向核心工作间（动物室），核心工作间（动物室）气流由相对清洁侧流向污染侧；送风采用粗、中、高三级过滤，排风采用一级高效过滤；生物安全柜单独排风；IVC动物笼具和负压柜的排风并入实验室排风系统；设置备用排风机。
Abstract：
With two examples of negative pressure biosafety level-2 laboratories, discusses the design of functional zoning and layout, ventilation and air conditioning modes, air distribution, air supply and exhaust systems. Offers following suggestions: to divide the core working room (or animal room) into the relatively clean side and the relatively polluted side, to arrange the buffer, dressing and preparation rooms, to use the all-air direct flow air conditioning system, to set the flow direction of the overall airflow from the clean area to the core working room (or animal room), and the airflow in the working room (or animal room) from the relatively clean side to the polluted side, to use the primary-intermediate-high efficiency three-stage filtration in the air supply system, and the one-stage high efficiency filtration in the air exhaust system, to design separate air exhaust in the biological safety cabinets, to incorporate the IVC and negative pressure cabinet exhaust as part of the laboratory exhaust system, and to set spare exhaust fans.
Keywords:biosafety level-2 laboratory, negative pressure, functional zoning, layout, ventilation and air conditioning, air distribution, air supply and exhaust system