Abstract：

Theoretically analyses and summarizes the influencing factors of isolation effects, including the pressure difference, door opening and closing, passing in and out of persons, temperature difference and buffer rooms. By establishing isolation wards with single bed and double beds, experimentally studies three schemes for supply and exhaust outlet positions and isolation effects of buffer rooms. Indicates that pressure difference can not counteract the effects of other factors, that making main flow region and applying directional air flow are primary measures for protecting doctors and nurses, that the effect is the best when adopting double supply outlets, with one in the bed side and the other in the bed end, that it is the most important dynamic isolation measure for setting buffer rooms to dilute and filtrate pollutants and selfclean.