屏障环境下换笼操作对局部洁净度影响的动态研究
Dynamic study on effect of cage changing operation on local cleanliness in barrier environment
摘要：
由于屏障环境洁净度参数在GB 14925—2010《实验动物 环境及设施》中是静态指标，为了探索屏障环境内动物饲育区动态空气洁净度变化规律及屏障环境污染原因，为作业指导书提出理论依据，故进行本实验研究。采用在线空气粒子计数器，对独立通风笼盒系统不同操作空间进行了洁净度评价和统计分析。结果显示，因换笼操作引发动物饲养室内发尘量剧增，洁净度降低。实践中，现行国家标准对运行状态下屏障环境内洁净度无具体要求，由于忽略洁净室产尘量少的基本原则，导致发尘量大增，洁净度降低。
Abstract：
Since the cleanliness parameter of the barrier environment is a static index in the GB 14925-2010 Laboratry animal—Requirements of environment and housing facilities, carries out the experimental study in order to explore the dynamic air cleanliness changes in animal feeding areas in the barrier environment and the reasons for the barrier environment pollution, and to provide a theoretical basis for the operating instructions. Using the online air particle counter, evaluates and statistically analyses the cleanliness of different operating spaces of the independent ventilation cage system. The results show that due to the cage changing operation, the amount of dust in the animal feeding room will increase sharply, and the cleanliness level will reduce. In practice, the current national standards have no specific requirements for the cleanliness of the barrier environment under operating conditions. Due to the neglect of the basic principle of low dust production in clean rooms, the amount of dust is greatly increased and the cleanliness is reduced.
Keywords:barrierenvironment,cleanliness,cagechangingoperation,laboratoryanimal,particleconcentration