Pressure variation trend in gas sterilization process in high-level biosafety laboratories

Taking a relatively airtight gas disinfection room in BSL-4 as research object, selects three disinfectants (hydrogen peroxide, formaldehyde and chlorine dioxide) and four disinfection equipment (hydrogen peroxide dry-mist vapor, hydrogen peroxide vapor, formaldehyde fumigator and chlorine dioxide gas fumigator). Each type of disinfection equipment uses a typical sterilization procedure to real-time monitor the room pressure and temperature changes during sterilization, and qualitatively analyse the causes of pressure variations. The results show that there is a positive pressure difference in the room during the process of sterilization. The main causes of room pressure variation during the process of sterilization are the increase of gas substance amount and the temperature variation. The positive pressure difference will lead to a leakage risk of pathogenic microbial at the initial stage of sterilization and damage risk of the envelope seal.